This week…in lab.
Whats going on this week? Right now I’m trying to create a menu of expression vectors containing an epitope tag with an IRES eGFP. What this means is that whatever gene I express using one of these vectors will have a special tag on one end or the other plus eGFP will show up wherever the gene does.
This is great for detection purposes because not only do the cells expressing my gene of interest fluoresce green, but I can probe for my tags as well. I wish they just sold these things online. I’ll post maps of these constructs, and if anybody wants to save themselves weeks worth of molecular biology, just send me a self addressed stamped envelope and I will send you an aliquat of any of my DNA. Oh. Good idea. I am going to start a construct gallery this weekend.