Oh HELLs yes!
After months of failure. I finally succeeded in electroporating my novel reporter gene into the brain of a newborn mouse. As it turns out the equipment I was using was crap, and since I don’t know anything about unipolar square pulses with exponential decay, I was unaware of its shortcomings. So after ~250 mice injected that sacrificed. Here is the result.
The champagne doesn’t pop just yet however. This wasn’t what I was going for. Getting GFP to express like this was an achievement a few years ago, but it is just the control for my real experiment. The question is, does this correlate to an MRI? I have a whole litter of these pups, and hopefully the other 9 have the same kind of expression as the 3 who were sacrificed so far.
But, if they really do correlate, suddenly it is endgame for my thesis and I can start doing some cool stuff with the my new technology.